Post-Doctoral Fellow in Directed Differentiation of Stem Cells
POSTED 10/02/2009
Objective
We seek a postdoctoral research scientist/engineer to work on a NIH-funded research project concerned with the directed differentiation of embryonic stem cells into endothelial cells for applications in tissue engineering vasculogenesis/angiogenesis. This research involves creation of novel reporter/selection genetic constructs, generation of stable embryonic stem cell lines, differentiation to endothelial cells and characterization, as well as implantation into animal models. An ideal candidate will have experience in stem cell culture and differentiation, particularly to endothelial cells; endothelial cell characterization; and basic molecular biology techniques (e.g. cloning, PCR, Western, immunohistochemistry, etc.).
Essential Functions
The successful applicant will be expected to:
• Differentiate stem cells to endothelial lineages according to specific protocols.
• Evaluate undifferentiated and differentiated cells (microscopy, FACS, etc.).
• Generate reporter constructs using molecular biology skills.
• Transfect and select clones.
• Grow and maintain fibroblast cells.
• Implant cells in animal models.
• Maintain a database of stored cells and plasmids.
• Maintain careful and detailed laboratory notebooks.
• Provide minimal teaching of others (basic cell culture techniques, cloning, etc.).
Requirements
Experience
Minimum of 3-5 years experience in biological research; experience with plasmid/construct transfection is required. Special consideration will be given to applicants with previous experience in stem cell culture or derivation (ES preferred), and differentiation to endothelial cells.
Education
PhD degree in Genetics, Molecular Biology, Biomedical Engineering or related disciplines.
Essential Skills
• Organization and careful laboratory notebook skills.
• Precise and reproducible attention to scientific detail.
• Basic computer skills (e.g. word processing).
Technical Skills
• Sterile technique.
• Cell culture skills.
• Basic cellular characterization (microscopy).
• Basic molecular biology skills (bacteria transformation, DNA isolation, restriction mapping, etc.)
Interested parties should send Curriculum Vitae and list of 3 references to:
Horst von Recum
Assistant Professor, Biomedical Engineering
Case Western Reserve University
10900 Euclid Ave.
Cleveland, OH 44106
horst.vonrecum@case.edu
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