Laboratories
The Experimental Mechanobiology Laboratory is located on the 5 th floor of the Wickenden Biomedical Engineering Building at Case Western Reserve University . It accommodates a cell and tissue culture lab (equipped with a Thermoforma, Stericycle CO2 controlled incubator, two free-standing laminar flow hoods for sterile cell culture, and cryostorage capabilities). In addition, the biochemistry wet lab is equipped with a Perkin-Elmer Fluoresence/Absorbance/Luminescence plate reader, digital filter fluorometer, pH and temperature meters, dessicators, hot plates and warm water baths, homogenizer, centrifuge, precision scales, and an ultra low temperature freezer. Contiguous to that space is a lab manager and student work area in which one engineer and four students have their desks. The greater lab accommodates an additional cell and  tissue culture imaging lab with a Leica DMIRE2 inverted microscope (equipped with bright field and fluorescence imaging applications, 4 position fluorescence registered turret and motorized xyz controls, allowing for precise and repeatable in situ colocalization and 3D rendering of fluorescent probe distributions and specimen architecture). Software support for these applications is provided through Improvision OpenLab and Volocity software packages which run on a MacIntosh G4-based platform. Additionally, the lab contains a BOSE technology enhanced EnduraTEC ELF3230 100lb high frequency response mechanical testing system.
The Computational Mechanical Biology Laboratory is housed in the Case School of Engineering's Computational Think-Tank for Multiscale Modeling of Biological and Bio-inspired systems , in the second level of the Olin Materials Science Building. It is equipped with 10 PC based workstations, 2 dedicated site licenses for Abaqus Finite Element Software used for computational modeling of poroelastic behaviour, fluid flow and mass transfer in musculoskeletal tissues, and one dedicated site license to CFD-Ace (a computational fluid dynamics software). The PI's computational laboratory is also outfitted with a G5-power PC (Macintosh) based imaging workstation.
The In Situ Imaging Laboratory is located on the 1 st floor of the Wicke nden Biomedical Engineering Building, across the hall from the Neuroengineering Surgical Laboratories . The so-called IISIS lab houses a custom configured Intravital and In Situ Imaging System , a microscope based on the Leica TCS SP2 AOBS Spectral confocal microscope system (Leica Microsystems, Mannheim , Germany ) and is situated to maintain precise environmental and surgical controls. A wet lab bench and cabinet is located in the lab for ongoing experiments. The microscope is outfitted with a high-end digital video camera ( Orca Extended Range , digital cooled) for image and video capture.
The Hard and Soft Tissue Histology Core is located on the 1 st floor of the Wickenden Biomedical Engineering Building at Case Western Reserve University. The laboratory houses standard paraffin- as well as hard tissue histology/cytochemistry equipment including a linear precision saw (Isomet 4000, Buehler), an Ecomet 4/Automet variable speed semi-automated grinder/polisher, a Leica SP1600 diamond saw microtome, and Wells Diamond Wire Saw, as well as fumehood for immunohistochemistry and one dedicated for polymethylmethacrylate embedding, an one explosion proof refrigerator for specimen and reagent storage.
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