Primary Faculty

Eben Alsberg, Ph.D.


Eben Alsberg, Ph.D.
Associate Professor

Office: Room 204 Wickenden Building
Phone: (216) 368-6425
Fax: (216)368-1509
Email: eben.alsberg@case.edu
Mail Address: Room 309 Wickenden Building 2071 Martin Luther King Jr. Drive Cleveland, OH 44106-7207

Selected links:

• Alsberg Stem Cell and Engineered Novel Therapeutics Lab (ASCENT)
• Eben Alsberg receives prestigious Ellison Medical Foundation New Scholar in Aging Award
Eben Alsberg named to Crain's Cleveland Business Forty Under 40

Research Summary

Our laboratory focuses on engineering functional biologic replacements to repair damaged or diseased tissues in the body. We use the complex signals that are implicated in tissue morphogenesis, repair, and homeostasis as a template for the development of innovative biomaterials for tissue regeneration. Through the precise temporal and spatial presentation of soluble bioactive factors, mechanical forces, and biomaterial physical and biochemical properties, we aspire to create microenvironments that regulate cell gene expression and new tissue formation. Some areas of active investigation include controlling stem cell differentiation, delivering bioactive factors sequentially, developing spatially patterned constructs, understanding cell-cell interactions, and determining mechanical influences on cell function.

Recent Publications

2014

Jeong SI, Burns NA, Bonino CA, Kwon IK, Khan SA, Alsberg E: Improved cell infiltration of highly porous 3D nanofibrous scaffolds formed by combined fiber-fiber charge repulsions and ultra-sonication. Journal of Materials Chemistry B (in press, 2014).

Nguyen K, Jeon O, Krebs MD, Alsberg E: Sustained localized presentation of RNA interfering molecules from in situ forming hydrogels to guide stem cell osteogenic differentiation. Biomaterials (in press, 2014).

Dang PN, Solorio LD, Alsberg E: Self-assembling human adipose-derived stem cell sheets incorporated with growth factor releasing biodegradable polymer microspheres for cartilage regeneration. Tissue Engineering A (in press, 2014).

Fu AS, Solorio LD, Alsberg E, Saidel GM: Mathematical modeling of glycosaminoglycan production by stem cell aggregates incorporated with growth factor-releasing polymer microspheres. Journal of Tissue Engineering and Regenerative Medicine (in press, 2014).

Yu X, Dang PD, Alsberg E, Murphy WL: Multilayer inorganic microparticles for tunable dual growth factor delivery. Advanced Functional Materials (in press, 2014).

Nguyen K, Alsberg E: Bioactive factor delivery strategies from engineered polymer hydrogels for therapeutic medicine. Progress in Polymer Science (in press, 2014).

Cheng C, Solorio LD, Alsberg E: Decellularized tissues for orthopaedic tissue engineering. Biotechnology Advances, 32(2):462-84, 2014.

Jeon O, Samorezov J, Alsberg E: Single and dual crosslinked oxidized methacrylated alginate/PEG hydrogels for bioadhesive applications. Acta Biomaterialia, 10(1):47-55, 2014.

Andersen T, Markussen C, Dornish M, Heier-Baardson H, Melvik JE, Alsberg E, Christensen BE: In situ gelation for cell immobilization and culture in alginate foam scaffolds. Tissue Engineering, Part A, 20(3-4)600-10, 2014.

Andersen T, Melvik J, Gåserød O, Alsberg E, Christensen B: Ionically gelled alginate foams: Physical properties controlled by type, amount and source of gelling ions. Carbohydrate Polymers, 99:249-56, 2014.

Heylman CM, Santosa S, Krebs MD, Saidel GM, Alsberg E, Muschler GF: Modeling and experimental methods to predict optimal oxygen distribution for tissue regeneration in bone defects Medical and Biological Computing and Engineering, 52(4):321-30, 2014.

2013

Jeon O, Alt D, Ahmed SM, Linderman S, Alsberg E: Biochemical and physical signal gradients in hydrogels to control stem cell behavior. Advanced Materials, 25(44):6366-72, 2013.

Jeon O, Alsberg E: Regulation of stem cell fate in a three-dimensional micropatterned dual-crosslinked hydrogel system. Advanced Functional Materials, 23(38):4765-75, 2013. Highlighted as Frontispiece.

Saquing C, Tang C, Monian B, Bonino C, Manasco J, Alsberg E, Khan S: Alginate-PEO blend nanofibers and the role of carrier polymer in electrospinning. Industrial & Engineering Chemistry Research, 52(26):8692-8704, 2013.

Jeon O, Alsberg E: Photofunctionalization of alginate hydrogels to promote adhesion and proliferation of human mesenchymal stem cells. Tissue Engineering, Part A, 19(11-12):1424-32, 2013.

Corn DJ, Kim Y, Krebs MD, Mounts T, Molter J, Gerson S, Alsberg E, Dennis JE, Lee Z: Imaging early stage osteogenic differentiation of mesenchymal stem cells. Journal of Orthopaedic Research, 31(6):871-9, 2013.

Solorio L, Vieregge E, Dhami C, Alsberg E: High density cell systems incorporating polymer microspheres as microenvironmental regulators in engineering cartilage tissues. Tissue Engineering, Part B, 19(3):209-20, 2013.

Nyugen K, Dang PN, Alsberg E: Functionalized, biodegradable hydrogels for control over sustained and localized siRNA delivery to incorporated and surrounding cells. Acta Biomaterialia, 9(1):4487-95, 2013.

2012

Andersen T, Melvik J, Gåserød O, Alsberg E, Christensen B: Ionically gelled alginate foams: Physical properties controlled by operational and macromolecular parameters. Biomacromolecules, 13(11):3703-10, 2012.

Jeong SI, Jeon O, Krebs MD, Hill MC, Alsberg E: Biodegradable photocrosslinked alginate nanofiber scaffolds with tunable physical properties, cell adhesivity, and growth factor release. eCells and Materials, 24:331-43, 2012.

Solorio LD, Vieregge EL, Dhami CD, Alsberg: Spatiotemporal regulation of chondrogenic differentiation with controlled delivery of TGF-β1 from gelatin microspheres in mesenchymal stem cell aggregates. Stem Cells Translational Medicine, 1(8):632-9, 2012.

Chim H, Miller E, Gliniak C, Alsberg E: Stromal-cell-derived factor (SDF) 1-alpha in combination with BMP-2 and TGF-β1 induces site-directed cell homing and osteogenic and chondrogenic differentiation for tissue engineering without the requirement for cell seeding. Cell and Tissue Research, 350(1):89-94, 2012.

Bonino CA, Efimenko K, Jeong SI, Krebs MD, Alsberg E, Khan SA: Three-dimensional electrospun alginate mats via tailored charge repulsions. Small, 8(12):1928-36, 2012.

Jeon O, Alt D, Ahmed SM, Alsberg E: The effect of oxidation on the degradation of photocrosslinkable alginate hydrogels. Biomaterials,33(13):3503-14, 2012.

Solorio LD, Vieregge EL, Dhami CD, Dang PN, Alsberg E: Engineered cartilage via self-assembled hMSC sheets with incorporated biodegradable gelatin microspheres releasing chondrogenic growth factor. Journal of Controlled Release, 158(2):224-32, 2012.

2011

Bonino CA, Samerozev JE, Jeon O, Alsberg E, Khan SA: Real-time in situ rheology of alginate hydrogel photocrosslinking. Soft Matter, 7(24):11510-17, 2011.

Lee JB, Jeong SI, Bae MS, Heo DN, Kim CH, Alsberg E, Kwon IK: Highly porous electrospun nanofibers enhanced by ultra-sonication for improved cellular infiltration. Tissue Engineering, 17(21-22):2695-702, 2011.

Jeon O, Powell C, Solorio L, Krebs MD, Alsberg E: Affinity-based growth factor delivery from biodegradable, photocrosslinked heparin-alginate hydrogels. Journal of Controlled Release, 154(3):258-66, 2011.

Bonino CA, Krebs MD, Saquing CD, Jeong SI, Shearer KL, Alsberg E, Khan SA: Electrospinning alginate-based nanofibers: From blends to crosslinked low molecular weight alginate-only systems. Carbohydrate Polymers, 85(1):111-119, 2011.

Jeon O, Krebs MD, Alsberg E: Controlled and sustained gene delivery from injectable, porous PLGA scaffolds. Journal of Biomedical Materials Research-A, 98A(1):72-79, 2011.

Krebs MD, Alsberg E: Localized, targeted and sustained silencing RNA Delivery. Chemistry – A European Journal, 17(11):3054-3062, 2011. (One of most accessed articles in 2011)

Jeong SI, Krebs MD, Bonino CA, Samorezov JE, Khan SA, Alsberg, E: Electrospun chitosan-alginate nanofibers with in situ polyelectrolyte complexation for use as tissue engineering scaffolds. Tissue Engineering: Part A, 17(1-2):59-70, 2011.

2010

Sasaki JI, Asoh TA, Matsumoto T, Egusa H, Yatani H, Akashi M, Alsberg E, Sohmura T: Fabrication of 3D cell constructs using temperature-responsive hydrogel. Tissue Engineering: Part A, 16(8):2497-2504, 2010.

Jeon O, Powell C, Ahmed SM, Alsberg E: Biodegradable, photocrosslinked alginate hydrogels with independently tailorable physical properties and cell adhesivity. Tissue Engineering: Part A, 16(9):2915-2925, 2010.

Jeong SI, Krebs MD, BoninoCA, KhanSA, AlsbergE: Electrospun alginate nanofibers with controlled cell adhesion for tissue engineering.  Macromolecular Biosciences, 10(8):934-943, 2010.

Solorio LD, Fu AS, Hernandez-Irizarry R, Alsberg E: Chondrogenic differentiation of human mesenchymal stem cell aggregates via controlled release of TGF-b1 from incorporated polymer microspheres. Journal of Biomedical Materials Research-A, 92(3): 1139-1144, 2010.

Krebs MD, Salter E, Chen E, Sutter KA, Alsberg E: Calcium phosphate-DNA nanoparticle gene delivery from alginate hydrogels induces in vivo osteogenesis. Journal of Biomedical Materials Research-A, 92(3): 1131-1138, 2010.

2009

Erb RM, Krebs MD, Alsberg E, Samanta B, Rotello VM, Yellen BB: Beyond diffusion-limited aggregation kinetics in microparticle suspensions. Physical Review E, 80(5 Pt 1), 2009.

Krebs MD, Jeon O, Alsberg E: Localized and sustained delivery of silencing RNA from macroscopic biopolymer hydrogels. Journal of the American Chemical Society, 131(26): 9204-9206, 2009.

Krebs MD, Erb RM, Yellen BB, Samanta B, Bajaj A, Rotello VM, Alsberg E: Formation of ordered cellular structures in suspension via label-free negative magnetophoresis. Nanoletters, 9(5): 1812-1817, 2009.

Krebs MD, Sutter K, Lin AS, Guldberg RE, Alsberg E: Injectable poly(lactic-co-glycolic) acid scaffolds with in situ pore formation for tissue engineering. Acta Biomaterialia, 5(8): 2847-2859, 2009.

Jeon O, Mansour JM, Alsberg E: Photocrosslinkable alginate hydrogels with tunable biodegradation rates and mechanical properties. Biomaterials, 30(14): 2724-2734, 2009.


 




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